Image Capturing Unit (Camera Head and Controller)
CCD chip: Fujifilm Super CCD Area Type chip
Number of Pixels: 3.2 M pixels
Pixel Size: 10.75 x 10.75µm
Cooling: Peltier
Cooling
Temperature: -30°C (when the room temperature is below 28°C)
-35°C (when the room temperature is below 20°C)
Focusing: Power focusing; remote and preset control
Exposure time: 1/100 second to 30 hrs (pre-set menu or manual input)
Dynamic range: 4 orders of magnitude
Gradation: 16 bits (65,536 gray levels)
Image size: 12.6MB Max.; 49.2KB Min.
Maximum sample
size: 25 x 25cm (wide view lens);
14 x 21cm (FUJINON VRF43LMD lens)
Binning: 1x2, 2x4, 4x8, and 8x16 pixels
Interface: USB1.1
Software
Image capture: Fujifilm Image Reader (Mac™ and Windows®)
Image analysis: Fujifilm Image Gauge (Mac™); Fujifilm MultiGauge (Windows®)
Dimensions and Weights
Camera head: 180 (W) x 170 (H) x 250 (D)mm 3.4kg
Dark Box IV: 510 (W) x 730 (H) x 480 (D)mm 49.0kg
Analyzing Unit
Operating system: Windows® XP or Mac™ OS 9.2.2, X.2, X.3
Applicable Reagents and/or Samples
Chemiluminescence:
CDP-Star®, ECL™, ECL Plus™, ECL Advance™, Lumi-Light Plus, Renaissance™, Bright-Star™, SuperSignal®, ImmunoStar, CSPD®
Fluorescence:
Blue LED: SYBR® Green I & II, SYBR® Gold, SYPRO® Ruby, SYPRO® Orange, SYPRO® Tangerine, FITC, FAM™, EGFP, ECFP, AttoPhos™
Green LED: SYPRO® Red, Cy™3, TMRA™, ROX™, HEX™, Alexa Fluor®532, Alexa Fluor®546,Deep Purple, Pro-Q™Diamond, Rhodamine Red™, BODIPY®576/589, NED™, R-phycoerythrin, RFP, HNPP
Red LED: Alexa Fluor®633, Alexa Fluor®635, Alexa Fluor®647, Cy™5, BODIPY®650/665, DiD, TOTO®-3, DDAO phosphate,
UV: Eidium Bromide, SYPRO®Rose, EtBr
Digitize: CBB/silver stained gel (Trans-illuminator)
NBT/BCIP stained membrane (EPI-illuminator)
Intelligent Dark Box IV
Blue-EPI-illuminator for fluorescence**: Blue LED (460nm)
Green-EPI-illuminator for fluorescence (option)**: Green LED (520nm)
Red-EPI-illuminator for fluorescence (option)**: Red LED (630nm)
EPI-illuminator for documentation: White-light source
White trans-illuminator for documentation:
of stained gels and Autoradiographic films: White LED
UV trans-illuminator***: UV-light source (312nm)
Filter turret: Five positions
Lens
High sensitivity lens (FUJINON VRF43LMD)
F-number: 0.85
Focal length: 43mm
Focus: Remote power focusing
Mount: Bayonet
Wide view lens
F-number: 2.0
Focal Length: 24mm
Focus: Manual
Mount: Adapter to Nikon F
(*No license is granted for use of AttoPhos™ to detect nucleic acid on a nylon membrane.
**White-light source for EPI-illumination is built-in in the same unit.
***No license is granted for pre-labeling gel electrophoresis method with the UV transilluminator.)
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