2D Gel of Sake Yeast Stained with SYPRO®Ruby
Fluorescent Stain
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(Ex. 473nm) Sample.
Courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research
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SYPRO®Orange Stained 2D Protein Electrophoresis
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Sample: Yeast
Courtesy of Mr. Yoshihiro Yamamoto, Kyoto Municipal Institute for Industrial Research
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SYPRO®Orange-stained Protein SDS-PAGE
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Sample: Bovine Serum Albumin
Applied Amount: MW Marker/1µg/500ng/200ng/100ng/50ng/
20ng/10ng/5ng/2ng/1ng/MW Marker
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SYBR®Green I stained Differential Display (SSDD)
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Sample: Mouse Testis
Courtesy of Dr. Yasuyuki Kurihara,Yokohama National University.
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EtBr-stained DNA Agarose Gel
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Sample: lambda DNA/Hind III
Applied Amount: 1µg/100ng/50ng/20ng/10ng/5ng/2ng/1ng
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SYBR® Green I-stained DNA in Agarose Gel
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Sample: lambdaDNA/Hind III.
Applied amount: 100ng/10ng/3ng/1ng/300pg/100pg/30pg /10pg
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Chemifluorescence Southern blotting. Detection with DDAO-phosphate 9H (1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)
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Sample:
Left four lanes: lambda DNA/Hind III
Right four lanes: lambda DNA/Eco RI+Hind III
Applied Amount:
Left four lanes: 10ng/20ng/50ng/100ng
Right four lanes: 10ng/20ng/50ng/100ng
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Chemifluorescence Detection of Southern Blotting. Detected with AttoPhos™ as
Alkaline Phosphatase Substrate
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Sample: pBR328/ Bam HI
Applied amount: 100pg/30pg/10pg/3pg/1pg/300fg/100fg/30fg/10fg/MW Marker
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Cy3™ and Cy5™ Fluorescent Dyes Processed on a
Single Sample
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Sample: Human lgG protein
Label: Cy3™ and Cy5™
Concentration: 100, 50, 25, 12.5, 6.25, 3.125, 1.56ug/lane
Excitation: Cy3™ 532nm, Cy5™ 633nm
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FITC(Fluorescein isothiocyanate):Conventional SDS-PAGE of
Protein
Sample: Monoclonal anti body labeled with FITC and MW marker labeled with FITC.
Method: SDS-PAGE
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FDD Method
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Sample: Xenopus
Label: Rhodamine labeled primer
Excitation: 532nm
Courtesy of the Laboratory of Molecular Gene Techniques, Kyushu University
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32P Label Macroarray Analysis of a Ready-made
Membrane (22x22cm), Human GDA ver1.2 Membrane (Genome Systems Inc.)
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BAS-MS IP 2340 was used and detected by FLA-3000.
Data courtesy of Dr. Norio Komatsu, M.D. Ph.D., Jichi Medical School and Dr. Masao Seto, M.D., Ph.D., Aichi Cancer Research Institute
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